After the image was focused, I then switched the gain back to 10db. I waited about 3 seconds to allow image to be displayed in preview screen when I turned the focus knob.), I had some visible image that I can use to focus. By setting with this gain and exposure time (i.e. I end up using 0.61 fps then adjust the gain to approximately 20 db and 3000 ms exposure time.
The light intensity of my DAPI stain was apparently not strong enough to use either 2.5 fps or 5 fps (in the Quick Fluo tab – in Advanced setting). I followed the instruction to use the focus mode then photo taking (fine) mode. After consulting the manual, I found out that there is a session written specifically for fluorescent microscopy. By default, TSView first loaded the auto white balance and auto exposure mode which are not intended for fluorescent microscopy. On the other hand, the operation of TSView software for taking fluorescent images is not as straightforward. I did not spend much time learning the software. It was good enough for me to focus the image easily. The live view is a little bit fuzzy but very responsive.
All I need to do is click on Live and Snap buton.
#TUCSEN CAMERA HOW TO#
It looks very intimidating initially but I can figure out how to do thing immediately. There are many setting that you can choose from.
#TUCSEN CAMERA SOFTWARE#
The Zeiss Axio Vision software is a very comprehensive image capturing and processing software. Vera Cell at 400X - Tucsen TCC-3.3ICE-N Exposure 3 sec
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